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Andrological Evaluation of Male Infertility pp Cite as. Nigrosin increases the contrast between the background and sperm heads, making sperm easier to visualize. Eosin stains only the dead sperm, turning them a dark pink, whereas live sperm appears white. This staining technique must be performed immediately after motility is assessed using sperm from the same semen sample.
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Eosin-Nigrosin Staining Procedure
Application of Two Staining Methods for Sperm Morphometric Evaluation in Domestic Pigs
Try out PMC Labs and tell us what you think. Learn More. The effect of two smear staining methods on the dimensions and shape of sperm cells in the semen of domestic pigs was evaluated. Each ejaculate was next sampled to make two microscopic slides, of which one was stained with eosin-nigrosin and the other with eosin-gentian dye. In total, measurements of sperm cells were made.
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